FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15589627
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA145450.016.6304879
AGAGCAC151600.015.8608598
GATCGGA164000.014.8697171
TCGGAAG206850.012.1122273
ATCGGAA212850.012.0096462
AAGAGCA210900.011.6742867
GAAGAGC286400.08.9235196
GGAAGAG291600.08.8137665
AGTCACA62300.07.92699425-29
CGGAAGA334550.07.63922834
CTAGTAC7251.4551915E-116.15711765-69
CAGTCAC86300.05.97275425-29
CTCGTAT85900.05.85001940-44
CACACGT89350.05.84959310-14
CCAGTCA91150.05.812909625-29
ACTAGTA9400.05.667970765-69
CACGTCT97250.05.463240610-14
GACTAGT9002.7284841E-115.4398965-69
TAGCTCT41554.7111826E-105.3989251
GTCACAG96800.05.38437925-29
Produced by FastQC (version 0.11.5)