FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15589627
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA168200.014.275531
AGAGCGT196650.012.1198688
TCGGAAG209150.011.5324863
ATCGGAA223300.011.2255142
GAGCGTC242250.010.2547759
AAGAGCG242700.010.1169567
GAAGAGC299850.08.4048366
GGAAGAG307450.08.3143075
CGGAAGA346050.07.4072484
TAGATCT66750.07.291004740-44
GTGCACT77150.06.38363630-34
AGAGTGT81650.06.24357625-29
GAGTGTG84100.06.14730525-29
AGTGTGC86300.05.89047425-29
GTAGGGA91050.05.78920515-19
GTACTAG9250.05.760349310-14
GTAGATC97050.05.50427740-44
TAGGGAA93150.05.488621715-19
TGTAGGG98600.05.448158315-19
TGTGCAC95000.05.39640330-34
Produced by FastQC (version 0.11.5)