FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16038951
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGGCTCATCTCGTAT290830.18132731997248447TruSeq Adapter, Index 12 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA192400.024.6211229
GATCGGA200600.024.531941
AGAGCAC196950.024.015768
TCGGAAG263000.019.025693
ATCGGAA284600.017.7121242
AAGAGCA286150.017.2087527
ACACTAT74400.016.0628328
AACACTA73350.015.99832257
GAAGAGC334050.014.9566736
CACTATA82200.014.6262049
GGAAGAG346850.014.6122335
ACCAAAC94600.013.6991213
CGGAAGA385800.013.1929784
AGTCACA84200.011.78218325-29
AAACACT105350.011.54886156
CCAAACA115050.010.8254294
CAAACAC116600.010.5580385
CAGTCAC98000.010.3140625-29
CCAGTCA115350.09.13717825-29
TACCAAA153700.08.9019932
Produced by FastQC (version 0.11.5)