FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16038951
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGCTGATAGTGTAGATCT308690.19246271155763242Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA206500.023.709111
AGAGCGT233650.020.6789938
GAGCGTC256200.019.0837339
TCGGAAG268100.018.503643
ATCGGAA288800.017.4828682
AAGAGCG280850.017.357497
ACACTAT72400.017.3054688
AACACTA74700.016.4834527
CACTATA77700.015.9396989
GAAGAGC340250.014.7081956
GGAAGAG350700.014.1878845
CGGAAGA389650.012.731614
ACCAAAC100000.012.4560643
AAACACT103750.012.284516
TAGTGTA84000.012.2228935-39
TAGATCT91450.011.19446340-44
CAAACAC115650.011.145075
CCAAACA111200.011.07210354
AGTGTAG91650.010.77727640-44
ATAGTGT108700.09.49846235-39
Produced by FastQC (version 0.11.5)