FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16759474
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTAACTCGTGTAGATCT249760.14902615678749823Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA153250.027.9857851
TCGGAAG221650.019.2595253
AGAGCGT227850.018.7352338
ATCGGAA232600.018.7310072
GAGCGTC255150.016.8439989
AAGAGCG257400.016.8081287
GAAGAGC340150.012.9095626
TAACTCG64150.012.83886435-39
GCTAACT67250.012.69685430-34
CTAACTC67100.012.55356530-34
GGAAGAG355700.012.4059135
CGGAAGA367050.012.0028274
TGCTAAC78500.011.04232530-34
GTGCTAA84100.010.42689530-34
TAGATCT87650.010.16993240-44
TGTGCTA94400.09.15193730-34
ACTCGTG94450.09.13171135-39
CTCGTGT97800.08.98086635-39
GTAGATC112150.08.30776840-44
ACTAATC43750.08.227329
Produced by FastQC (version 0.11.5)