FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12731271
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA70950.023.4394515
CAGTGGT80050.020.7757764
GTGGTAT97950.017.4192736
GTATCAA116050.014.9505819
TGGTATC123300.014.2466557
AGCAGTG124100.013.8109772
GGTATCA130150.013.2755728
AACACTA38550.012.8857857
GCAGTGG135850.012.6687953
CACTATA39350.012.6238119
ACACTAT39400.012.6077918
AAGCAGT149100.011.9393411
AAACACT75900.06.82931046
GCCCTTA46450.06.5098384
TATTTAG51400.06.16276659
ACCAAAC77450.05.95052963
CAAACAC98500.05.11622525
TACCAAA101350.05.11596972
Produced by FastQC (version 0.11.5)