FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12731271
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA88300.016.0607227
ATGGCTC95750.014.6604925
TGGCTCA103400.014.1330216
CTCAAAC103450.013.9870179
AACACTA36450.012.4423537
CACTATA38500.011.9668219
TCTGATG123300.011.7826711
ACACTAT40800.011.64510258
GCTCAAA138350.010.5627358
GATGGCT135400.010.4205374
CTCGGGT37350.07.55528620-24
CTGATGG227350.06.55445532
TGATGGC244550.06.53488643
AAACACT74750.06.45242746
ACCAAAC74350.05.22835973
Produced by FastQC (version 0.11.5)