FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12895160
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTCTTCAGTGTAGATCT198210.1537088333917532Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA178100.019.1772141
TCGGAAG196450.017.598613
ATCGGAA224300.015.4805992
AGAGCGT227600.015.2210668
AAGAGCG264400.013.3749557
GAGCGTC271100.012.7481139
CGGAAGA289100.012.0832224
GGAAGAG321250.011.0305615
TAGATCT65750.010.92835540-44
GAAGAGC355400.010.1935686
AGTGTAG68250.010.06388740-44
CAGTGTA72150.09.93884635-39
TCAGTGT82600.08.90807335-39
GAGTGTT81900.08.82595525-29
AGTGTTC91600.07.8598925-29
AGAGTGT93100.07.7487225-29
TTCAGTG97550.07.557630535-39
GTAGATC101400.07.54061240-44
TCATTAA95650.07.436918365-69
TGTAGGG99150.07.40676415-19
Produced by FastQC (version 0.11.5)