FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14696327
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA181700.09.7511831
TCGGAAG205100.08.8538343
AGAGCGT206500.08.513488
GAGCGTC232550.07.83865459
ATCGGAA235350.07.8344382
AAGAGCG243550.07.3958697
GTGTCTA54350.06.54296325-29
TGTGTAG56850.06.484684540-44
TAGATCT61650.06.283455440-44
GGAAGAG295950.06.23245245
GAAGAGC296150.06.1795856
CGGAAGA322600.06.0096544
CTGTGTA75950.05.34682835-39
Produced by FastQC (version 0.11.5)