FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19605288
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAC126450.021.3424229
TGGCTCA136450.020.3066046
TCTGATG139800.019.2719941
ATGGCTC148550.018.5072065
GGCTCAA156700.017.4986237
GCTCAAA157100.017.3624488
GATGGCT207050.013.282614
CCTCTAG10700.011.16367250-54
CTGATGG295750.09.4721282
TGATGGC349450.08.5308783
CTAGCAC10550.07.77562350-54
AACACAG88200.06.62504110-14
CTCTAGC11600.06.57552850-54
CTCTAGG7600.06.43840250-54
ACACAGC100750.05.942640310-14
TCTAGCA12650.05.91596550-54
CTCGGGT110900.05.45043520-24
TCTAGGA9205.0931703E-115.318680350-54
CTAGGAT10002.0008883E-115.181020350-54
GGGTGGG115800.05.17017625-29
Produced by FastQC (version 0.11.5)