FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20043105
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA93800.021.7177375
CAGTGGT104100.019.372054
GTGGTAT132250.015.6216896
GTATCAA150600.014.0532989
AGCAGTG156250.013.3721522
GGTATCA165650.012.8198258
TGGTATC166050.012.5718997
GCAGTGG177000.011.6782273
AAGCAGT205000.011.1427391
CACTATA55800.08.3856179
AACACTA61050.08.3717167
ACACTAT65900.07.64652548
ACCAAAC102400.05.83776243
AAACACT99050.05.3779786
Produced by FastQC (version 0.11.5)