FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19611861
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT99700.023.8556234
AGTGGTA107800.022.5703415
AGCAGTG149550.016.7735822
GTGGTAT153600.015.9809786
GCAGTGG170500.014.1204813
TGGTATC185850.013.4015097
AAGCAGT203100.012.7772281
GTATCAA206000.012.5096759
GGTATCA204600.012.2084258
CACACTA31300.010.8092037
ACACACT54350.06.887226
CTAGCTA11500.06.63499325-29
ATCTAGC13800.06.15510325-29
ACACTAT62650.05.8598288
CACTATA64200.05.71827949
AACACAC78550.05.0403035
Produced by FastQC (version 0.11.5)