FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16177135
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA54100.015.8334449
GCCACTC64450.013.5140498
AACACTA64650.09.352637
ACACTAT66850.09.1525168
AGCCACT98350.09.0023057
AAGCCAC100650.08.8682996
CACTATA84750.07.04959349
CCGTTAA63850.06.8781434
AAACACT92650.06.6816766
CTCACTA29550.06.38210415-19
CCAAACA103100.06.35455234
CGTTAAA61250.06.34619435
ACAAAGC162000.06.2332543
TACAAAG172550.06.22835162
CAAACAC97400.06.06011155
ACCAAAC109150.05.418663
ATACAAA192050.05.38261371
Produced by FastQC (version 0.11.5)