FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16670715
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC167700.10059556533717959No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA123800.031.1104055
CAGTGGT124000.030.886154
AGCAGTG145700.027.0820872
GTGGTAT171950.022.4406346
AAGCAGT179450.022.258821
GCAGTGG186000.021.1718433
GTATCAA211800.018.862279
TGGTATC218050.018.0594187
GGTATCA218050.017.9584878
GAGCACA151500.012.8285659
AGAGCAC156650.012.4068158
GATCGGA176450.011.2980151
AAGAGCA233850.08.6505057
TCTTGGG94250.08.63082235-39
TCGGAAG249050.08.3829283
ATCGGAA285650.07.7134442
ACCTAAG51500.07.687746530-34
GAAGAGC283150.07.347746
CCTAAGG56450.07.090134630-34
CTAAGGT61550.06.57282230-34
Produced by FastQC (version 0.11.5)