FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16670715
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA117950.029.4786647
CTCAAAC125950.027.8363889
ATGGCTC133900.025.9139085
GCTCAAA155850.022.6807178
TGGCTCA160100.021.9430796
TCTGATG184250.018.69941
GATGGCT210900.016.9303674
CTCTAGG14300.016.41103650-54
CCTCTAG19150.015.9386550-54
TCTAGGA19100.012.81445150-54
CTAGGAT20000.012.16581350-54
CTCGGGT61450.011.26933520-24
GATCGGA178200.010.9191341
CTGATGG338100.010.6681792
TGATGGC350400.010.4785823
GAGCGTC212350.09.3570499
AGAGCGT207950.09.3473168
GGTCCTC74600.09.30217245-49
AAGAGCG218700.09.1836237
AGGTCCT84400.08.25617745-49
Produced by FastQC (version 0.11.5)