FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13083012
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC2220231.6970327627919322No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG896890.6855378562673489No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA544040.4158369647601026No Hit
AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA427550.3267978352385521No Hit
CTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT141660.10827781859406686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGAGAC56650.014.7603542
TGGGGGG41100.011.8567881
AGAGACG72500.011.434013
AGGGGGG32650.010.6928171
GGGGGGG73500.010.6900042
GATCGGA166350.08.0450551
GGAGAGA125800.07.51617341
GGGTGGG63000.07.3228162
GAGCACA189750.07.2452359
AGAGCAC191750.06.98197758
GGGGGGA39050.06.82997942
TCGGAAG207100.06.6480153
GGGAGGG83550.06.64328672
GACTAGG11600.06.5788207115-119
GGGGGGT27551.4242687E-96.5411952
ATCGGAA231050.06.33328152
GGGGTGG86300.06.06814241
GAGACGA68850.05.95984364
TCCTCTT458550.05.9185954
ACTAGGT11800.05.735166115-119
Produced by FastQC (version 0.11.5)