FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13059754
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC353770.270885653742023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCATG28900.011.7059258
GGGGGGG103400.09.5494432
TACTTAC56050.09.5155553
ACTTACC59100.08.7699194
GGGTGGG72400.08.4617162
TGGGGGG40400.07.91581771
GTTACTT77150.07.72506951
CCGGGTA81500.07.2420579
TTACTTA79400.07.1710812
GTGAGTA33450.07.11043022
CCACTCA36750.07.05100259
CTCGCCA82400.06.70364241
AGTGAGT30604.0017767E-116.65060471
GGGGGGA65850.06.5670982
GGGGTGG99850.06.478381
GGGAGGG104900.06.3897742
AGGGGGG52600.06.35618541
TAAGGGG31055.0386006E-106.2590429
GTCCCTT62150.06.1981091
CCTTTAG52550.06.1643944
Produced by FastQC (version 0.11.5)