FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13059754
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG221280.16943657591100109No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA144310.11049978429915296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCATG31600.011.390428
ACTTACC57200.07.55199964
TACTTAC57200.07.17439943
CCTTTAG53850.06.81853634
CCACTCA38950.06.46870579
GTCCCTT64150.06.20249371
TAAGGGG30852.8285285E-96.06701479
GGGTGGG66750.05.82870342
GGGGGGG98450.05.7629421
AGTGAGT29208.1770304E-75.55148361
TTAGCAT72800.05.53728447
CCGGGTA85800.05.4535959
GTGAGTA33158.412826E-85.43357852
CTCGCCA84700.05.39358471
GTTACTT80600.05.3022741
ACACTCT47305.820766E-115.2964761
AGGGGGG51252.0008883E-115.17580461
CCCTTTA70950.05.17516853
GAGCCCA38557.899689E-85.04192359
CTTTAGC84300.05.0379985
Produced by FastQC (version 0.11.5)