FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13726522
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGACTGGAATCTCGTAT324250.23622152792965326TruSeq Adapter, Index 23 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA180950.031.164571
AGAGCAC207400.027.1059788
AGTGGTA95100.026.8701845
GAGCACA210150.026.8540299
TCGGAAG211250.026.7482723
ATCGGAA249000.022.9796892
CAGTGGT113300.022.6174134
AGCAGTG119850.021.5863592
GTGGTAT130400.020.1482856
CGGAAGA306250.018.615384
AAGAGCA331550.017.1948727
GTATCAA158800.016.499619
GAAGAGC360700.015.8850926
GGAAGAG371550.015.7505635
GGTATCA177750.015.0240548
AAGCAGT174850.014.8982551
TGGTATC182000.014.5545647
GCAGTGG199450.013.3894473
CAGTCAC110050.010.49208525-29
CTCGTAT115850.09.88047840-44
Produced by FastQC (version 0.11.5)