FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14497137
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA96400.018.523557
TGGCTCA108400.016.406556
ATGGCTC113350.015.943895
CTCAAAC116000.015.8891839
TCTGATG130950.014.0940341
GCTCAAA154300.011.8057938
GATGGCT162400.011.1282474
AACACTA43650.08.7426117
CTGATGG230450.08.4689982
CACTATA48200.08.0661519
ACACTAT47300.07.91558078
TGATGGC256550.07.91433953
CCTCTAG17100.06.904086650-54
CTCTAGG12150.06.75440450-54
CTCGGGT53250.06.597103620-24
GGTCCTC72700.05.16869445-49
AAACACT83950.05.14612256
Produced by FastQC (version 0.11.5)