FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18904180
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCACTA72050.039.3598638
CACCACT94500.029.9331257
CCACTAC96200.029.4789819
ACAACAC134700.021.4996453
ATACAAC136650.021.1139281
ACGTTAA42150.019.8259164
ACACCAC159700.018.433586
TACAACA170700.017.4296782
CCTAGAC35600.015.771889120-124
GTTACGT55300.015.3061271
TACGTTA56900.014.9397243
ATCCTAG38650.014.861728115-119
AACACCA205500.014.4653695
CTAGACC40300.014.003936120-124
CTAACAC45000.012.86257625-29
TCCTAGA45800.012.698773115-119
CAACACC254400.011.9500074
CCCTAAT49150.011.687627125-129
TTAGGTC51450.011.67038245-49
CCTTAGG52900.011.24161545-49
Produced by FastQC (version 0.11.5)