FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18904180
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCACTA70450.036.675318
CCACTAC95650.027.3137919
CACCACT96950.027.021497
ACAACAC135000.019.603173
ATACAAC135000.019.438361
ACGTTAA43050.017.4202424
TACAACA169200.015.8115932
ACACCAC168400.015.6850756
CCTAGAC33400.015.260053120-124
ATCCTAG36800.014.51388115-119
GTTACGT57950.013.7763871
AACACCA200550.013.4650855
CTAGACC37750.013.4634695120-124
TACGTTA59400.013.1108653
TCCTAGA43950.012.152691115-119
CTAACAC46450.011.59175825-29
CCCTAAT48650.010.7720585125-129
TTAGGTC51200.010.73976445-49
CAACACC257600.010.7212874
GGGCCTA53200.010.22979685-89
Produced by FastQC (version 0.11.5)