FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21428188
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA103400.027.2920635
CAGTGGT128450.021.913594
GTGGTAT164250.017.6194176
GTATCAA173300.017.0301959
AGCAGTG185850.015.7678552
GGTATCA195750.014.9299588
TGGTATC201750.014.3087487
AAGCAGT240900.012.5574991
GCAGTGG233250.012.3456293
TCTTGGG121550.05.282981435-39
Produced by FastQC (version 0.11.5)