FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16718265
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA137550.020.363097
CTCAAAC151650.018.705349
ATGGCTC152650.018.2541265
TGGCTCA162600.017.0045076
TCTGATG184650.014.5582081
CCTCTAG13550.014.23810650-54
GCTCAAA207500.013.5325728
GATGGCT213800.013.2015194
CTCTAGG11350.013.0655650-54
CTAGGAT14550.010.29098750-54
TCTAGGA14400.010.09823850-54
GATCTAG6456.673727E-410.0466943
CTGATGG297200.09.54754452
TGATGGC325450.08.7609513
CTCGGGT67700.08.03891420-24
GGTCCTC95600.06.340077445-49
AGGTCCT97200.06.146843445-49
AACACAG104950.05.940472610-14
GGGTGGG102900.05.876518225-29
TCGGGTG103350.05.80913925-29
Produced by FastQC (version 0.11.5)