FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17287010
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA67750.013.59876259
GCCACTC81150.011.1758558
GAGCACA215850.08.5032879
AGAGCAC220400.08.2624278
GGCCTAA23450.08.16580775-79
AGCCACT110300.08.0265397
AAGCCAC116800.07.7031076
GATCGGA266350.07.34964941
TCGGAAG262050.07.2032223
CTCACTA29900.06.740422215-19
AAGAGCA304250.06.2692287
TACAAAG168400.06.07513482
ATCGGAA348250.06.0202092
GAAGAGC319650.06.0122266
TTAGGGG34850.05.741806105-109
AACACTA40956.366463E-115.6246417
ATTAGGG31600.05.6034374105-109
CCCCTAA35550.05.5885367110-114
ACACTAT49051.0913936E-115.2827768
ATACAAA230300.05.24067931
Produced by FastQC (version 0.11.5)