FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15992504
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC922770.577001575237999No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG579110.36211340012795995No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGAATCATCTCGTAT454190.28400180484557014TruSeq Adapter, Index 9 (97% over 38bp)
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA421220.263385896292413No Hit
AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA288780.18057209802792606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA237400.032.4023441
GAGCACA262050.028.9126159
TCGGAAG268600.028.7829723
AGAGCAC265300.028.5584268
ATCGGAA302550.025.7672022
CGGAAGA365950.021.2422244
AAGAGCA368100.020.8365767
GGAAGAG427550.018.2252645
GAAGAGC431050.017.9437456
ATTGTAC102400.017.215099
TAATTGC97600.014.0804265
CTACATT136150.013.6871945
ACATTGT152200.012.8111957
TACATTG154300.012.3103476
AATTGCC111100.012.17519956
ACAGGGG65500.011.9779694
TATCTAC158850.011.4195592
AGTCACG136950.011.30670625-29
CATTGTA174950.010.7753288
TTGCCTC129400.010.6204348
Produced by FastQC (version 0.11.5)