FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15992504
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG935390.5848927722638055No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA737140.4609284449764715No Hit
AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA510960.31949968560270486No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCGTAGCGTGTAGATCT465110.2908300038568069Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA257700.029.621511
TCGGAAG289650.026.2215613
AGAGCGT308900.024.727018
ATCGGAA319400.024.0272792
GAGCGTC324750.023.6753449
AAGAGCG343300.022.270247
CGGAAGA386950.019.869424
ATTGTAC103950.017.6599669
GAAGAGC442600.017.5664966
GGAAGAG453000.017.3697995
TAATTGC94600.014.76335
CTACATT140550.013.2660535
AATTGCC113850.012.8361816
TAGCGTG122700.012.4150635-39
ACATTGT156950.012.1551067
TACATTG154500.011.9750536
ACAGGGG68550.011.9719844
TTGCCTC127850.011.6558468
TATCTAC167200.011.1518212
CATTGTA174850.011.0754358
Produced by FastQC (version 0.11.5)