FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12729263
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG250350.19667281601456424No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA175950.13822481317260865No Hit
AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA149210.11721809817269076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC170750.08.7690669
AGAGCGT190000.07.99428378
GATCGGA202850.07.5215411
AAGAGCG214900.07.16832737
TCGGAAG253650.06.2729413
GAAGAGC255450.06.2276886
ATCGGAA282000.05.69751362
Produced by FastQC (version 0.11.5)