FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13416283
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCTC91050.013.1234385
GGCTCAA98400.012.4361127
CTCAAAC106000.012.1556449
TGGCTCA97550.011.9541476
TCTGATG109350.010.8648131
CCTCTAG10050.010.31399550-54
GATGGCT124750.09.924624
CTCTAGG7900.09.84072750-54
CTAGGAT9250.09.02706950-54
TCTAGGA9500.08.63797150-54
GCTCAAA149600.08.3723728
CTGATGG202000.06.52696232
TGATGGC235550.06.0201753
Produced by FastQC (version 0.11.5)