FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15346174
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG85000.06.846641
GTCTCCA89900.06.3925451
GTCTATT74500.06.2492821
GTCTTCA150800.06.0782091
GTCTACA67700.06.0173571
GTCTCCG143150.05.79321861
GTCCTAA22801.8525001E-55.74306961
GTCCCCA61700.05.6592971
GTAACTA38007.992639E-95.3601981
GTCCCTT70750.05.3466691
GTCTTCC139150.05.3324081
GTACCCG91350.05.2558361
GTCCTTA49154.5474735E-115.18025261
GTCTGCA162100.05.160851
GTCTGTG80450.05.15412861
GTATGTA69250.05.14733841
GTACCTG79600.05.11777731
GTCCTTT150850.05.1117191
GTCTGTA59800.05.1092191
GTCTTTC191800.05.0823271
Produced by FastQC (version 0.11.5)