FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14266779
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTCCAAATCTCGTAT207480.145428761460453TruSeq Adapter, Index 8 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT91000.023.810534
AGCAGTG91950.023.4968622
GCAGTGG102500.020.9986463
AGTGGTA109250.020.162485
GTGGTAT152050.014.5817026
AAGCAGT172100.012.0519371
GTATCAA193500.011.3092429
GATCGGA316550.010.7596061
GAGCACA326800.010.6391279
AGAGCAC330350.010.5683788
GGTATCA215650.010.3479218
TGGTATC218800.010.1989467
TCGGAAG357550.09.583283
ATCGGAA418150.08.6114882
CGGAAGA458250.07.8700814
GAAGAGC494300.07.3543576
AAGAGCA484550.07.2794477
GGAAGAG547850.06.63555
GTCACAC111500.06.352874325-29
TAGATCG75400.06.28318140-144
Produced by FastQC (version 0.11.5)