FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16824836
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT88550.018.2086564
AGTGGTA93100.017.4733925
GATCTAG5253.9583028E-715.0817543
CTAGCCG7958.731149E-1114.4867636
AGCAGTG121050.013.8112312
TCTAGCC8001.4933903E-913.4964565
GTGGTAT130500.012.6863236
GTATCAA149850.011.8647539
GCAGTGG147700.011.4039123
GGTATCA156250.011.3787728
TGGTATC146950.011.3641487
AAGCAGT157750.010.888111
ATCTAGC7502.1461958E-510.5572284
CAACTAA42600.06.75878867
CCTAATG42450.06.515021
CTAATGA57950.05.222822
ACTAATT58700.05.1502669
Produced by FastQC (version 0.11.5)