FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16824836
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAG4751.2689816E-716.6699773
GGCTCAA116900.012.8082927
TGGCTCA116400.012.6777456
CTCAAAC121300.012.2843449
CCTCTAG10250.011.23662850-54
ATCTAGC7802.737872E-611.0744614
ATGGCTC133000.010.9867715
TCTGATG148250.010.2358871
CAACTAA43800.09.203577
CCTAATG43050.09.025111
CTCTAGG10150.08.93602550-54
GCTCAAA176800.08.7131248
GATGGCT177250.08.4878174
TCTAGGA11250.08.0622850-54
CTAGGAT11250.07.806334550-54
CTAGCCG9700.00319188327.42111876
TCTAGCC9900.00389708937.27093845
CTAATGA58950.07.0889472
CTGATGG267450.06.16922242
ACTAATT64000.06.073749
Produced by FastQC (version 0.11.5)