FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19589227
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGCTGATAGTGTAGATCT344470.17584665285669518Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA174750.029.0839737
CTCAAAC194600.027.2285489
ATGGCTC188650.026.597725
TGGCTCA227400.022.5722146
GCTCAAA250050.020.7298018
GATGGCT250950.019.9658724
TCTGATG250000.019.5428031
GATCGGA314250.017.6651331
GAGCGTC317500.017.4823659
AGAGCGT338600.016.5417778
CCTCTAG28550.016.44002250-54
TCGGAAG359300.015.4887063
AAGAGCG366250.015.4297287
CTGATGG342900.014.9907572
CTCTAGG23400.014.70526750-54
TGATGGC392350.013.50507453
ATCGGAA429150.013.3703282
CTCGGGT77200.013.25961820-24
CTAGGAT26950.013.24902250-54
GAAGAGC444800.012.915566
Produced by FastQC (version 0.11.5)