FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16023366
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA62300.029.6952275
CAGTGGT67350.027.3617534
GTGGTAT101600.018.492196
AGCAGTG108750.017.7473742
GTATCAA111450.017.4388759
GGTATCA118150.015.9625528
GCAGTGG125600.015.0159633
TGGTATC129000.014.7317067
AAGCAGT145200.014.0365341
GTCCCTA25800.012.1315771
CTAGGGG5700.07.579238495-99
TCCCTAC47500.07.27742
ACTAGGG4951.3096724E-107.273986390-94
CCCTACC53200.06.630193
CACTAGG6207.094059E-116.50435290-94
CCTACCA55350.06.24259474
GAGCACA123850.06.04466349
GCACTAG6755.7661964E-105.974367690-94
CTAGATC8801.4551915E-115.5636306140-144
TTAGCAG32202.1248343E-75.4253011
Produced by FastQC (version 0.11.5)