FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16023366
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCTC99050.012.28465
TGGCTCA105500.011.8750036
GGCTCAA124200.010.3769177
CCTCTAG8000.010.25803650-54
CTCTAGG6000.010.07807150-54
TCTGATG134150.09.6105951
GTCCCTA25000.09.3764471
CTCAAAC138200.09.2209089
GATGGCT138150.08.9640934
ACTAGGG5900.08.7861490-94
CACTAGG6700.08.381803590-94
CTAGGGG5800.08.19205195-99
TCTAGGA8200.08.07650350-54
GCACTAG7600.07.389221790-94
CTAGGAT8400.07.37001850-54
GCTCAAA214950.06.16310648
CCCTACC52150.05.79857253
CTGATGG248900.05.49730352
TGATGGC261150.05.37614153
TCCCTAC50353.6379788E-125.2920422
Produced by FastQC (version 0.11.5)