FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19559361
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCGTTAATCTCGTAT349710.1787941845339426TruSeq Adapter, Index 10 (97% over 37bp)
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC260800.13333768930385814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA274500.022.0174739
AGAGCAC280050.021.5811358
GATCGGA290050.020.8778111
TCGGAAG333000.018.3319993
ATCGGAA381000.016.3247242
AAGAGCA396100.015.5487747
GAAGAGC408250.015.0330756
GGAAGAG463450.013.58404355
CGGAAGA464100.013.524564
GCGTTAA124850.09.98172135-39
CTCGTAT121050.09.97115540-44
CGTTAAT125150.09.79681335-39
GTTAATC134300.09.26864435-39
TCGTATG144550.08.6216645-49
TCTCGTA149250.08.34803440-44
AGCGTTA149100.08.27140535-39
AGTCACC165050.07.62910425-29
CAGTCAC174800.07.294126525-29
AATCTCG175400.07.160994540-44
CGTATGC189950.06.95143945-49
Produced by FastQC (version 0.11.5)