FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16318320
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA77200.017.9954195
CAGTGGT78400.017.6281684
AGCAGTG122600.011.8137982
GTGGTAT124250.011.6445216
GCAGTGG135950.010.8012233
TGGTATC146050.010.4485567
GGTATCA146400.010.325248
GTATCAA144500.010.2617479
AAGCAGT172650.08.810871
CTAGACG6551.7807906E-95.9349637120-124
CCTAGAC5605.7066427E-75.656272120-124
CCTAGTC6454.6855348E-85.58258880-84
GCTAGAC8458.385541E-105.282027120-124
CTAGTCT7351.4429861E-75.09496780-84
Produced by FastQC (version 0.11.5)