FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15552750
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCCTAAATCTCGTAT193500.124415296330231TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT105500.044.147074
AGCAGTG115800.040.2413022
GCAGTGG125400.037.5431143
TAGACCC32050.030.0961888
AGTGGTA169450.027.8259375
TTAGACC34200.027.7834857
GTGGTAT187850.025.1770156
AAGCAGT206200.022.9463981
GTTAGAC42950.022.4590686
TGGTATC217250.021.9349757
GGTATCA230150.020.9868018
AGACCCC52450.019.2139769
GTATCAA267900.018.1907549
TAGTTAG59350.016.980774
GAGCACA221650.014.9391139
AGAGCAC232900.014.2174938
GATCGGA238500.014.1923511
TCGGAAG274350.012.6471343
AGTTAGA86200.011.4409855
ATCGGAA303350.011.3728112
Produced by FastQC (version 0.11.5)