FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15552750
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATTCATCGTGTAGATCT198100.12737297262541994Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCC36450.036.741798
TTAGACC39200.033.981987
GGCTCAA114400.031.4707457
GTTAGAC45850.029.0532956
AGACCCC55750.024.1502729
CTCAAAC152100.023.8575949
TAGTTAG58150.023.5259594
TGGCTCA163350.022.613166
ATGGCTC182050.020.2106675
GCTCAAA192200.019.1431058
GATGGCT219400.016.6713434
AGTTAGA89350.015.3112115
TCTGATG239800.015.0919981
GAGCGTC245350.013.6455869
GATCGGA243250.013.4022011
TGATGGC293050.012.604233
AGAGCGT264000.012.6003598
CTCGGGT58600.012.30910320-24
TCGGAAG280800.012.0258683
CTGATGG320900.011.6258962
Produced by FastQC (version 0.11.5)