FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15765843
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT96200.029.3327394
AGTGGTA114600.024.8743745
AGCAGTG131700.021.6556632
GTGGTAT154200.018.4864046
GCAGTGG152100.018.4576763
TGGTATC164050.017.9467567
GGTATCA173400.016.8544458
AAGCAGT181350.015.8549411
CTAGGTC6501.1641532E-915.5044473
AGTGAGT42350.015.29754451
GTATCAA190650.015.1784269
GTGAGTA47650.014.056662
TAACCCC52900.012.2468489
CTCTAGG11753.6470046E-68.6731191
ATAACCC85200.07.85743578
GAGTATA93550.07.3870174
TCTAGGT14004.3588494E-57.20214842
GTATAAC98700.07.00157456
TGAGTAT106500.06.9619233
GTCTCTT81550.06.7838271
Produced by FastQC (version 0.11.5)