FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16533077
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT94750.037.151934
AGCAGTG117900.030.299752
AGTGGTA120400.029.3566675
GCAGTGG131550.026.8684353
GTGGTAT155750.022.9710086
AAGCAGT174500.020.1467761
TGGTATC190400.019.0171837
GGTATCA208300.017.2792388
GTATCAA235750.015.3894379
GAGCACA207850.07.37689269
AGAGCAC217050.07.16370738
ATCACAC79150.06.63920078
CAACGCA130150.06.3937710-14
GATCGGA256500.06.27131751
TCTTGGG123500.05.94546835-39
GCAGAGT133550.05.78897515-19
TCGGAAG270550.05.77382953
AACGCAG140000.05.707410-14
AATCCCC117000.05.6603839
TCACACC94400.05.41414939
Produced by FastQC (version 0.11.5)