FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16533077
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA130100.020.8645767
CTCAAAC146500.018.5764569
TGGCTCA164600.016.7100986
ATGGCTC168300.016.4280015
GCTCAAA180600.015.2689138
TCTGATG205150.012.9638211
GATGGCT240650.011.6386864
CTGATGG271400.010.0567192
TGATGGC325050.08.882433
CTCGGGT64950.08.44564820-24
ATCACAC82000.07.55112558
GGGTGGG82250.06.914273725-29
TCACACC91300.06.62395249
CCTCTAG27450.06.608730350-54
ACACAGC90900.06.54190710-14
GATCGGA256300.06.26027541
GGTGGGA95500.05.970039425-29
AACACAG103300.05.937825710-14
AGAGCGT270750.05.93012058
AATCACA109500.05.91798547
Produced by FastQC (version 0.11.5)