FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18605432
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTCTTCAGTGTAGATCT195520.10508758947386979Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA215650.013.5537571
TCGGAAG280050.010.6686823
ATCGGAA305250.010.1421152
AGAGCGT318650.09.4885738
GAGCGTC327550.09.098799
CCCTAAG60550.08.7983156
TACCCTA63600.08.60263254
AAGAGCG360050.08.3975397
CTAAGAG62100.08.34653958
CGGAAGA403050.07.60896544
GTATACC91050.07.501171
GGAAGAG478100.06.97169455
ACCCTAA79500.06.791575
TAGATCT97300.06.451688340-44
GAAGAGC529500.06.21344046
CCTAAGA93250.05.86719237
AGTGTAG114600.05.55312540-44
CAGTGTA119600.05.48950835-39
TAAGAGC98050.05.4330679
GTAGATC121450.05.37032340-44
Produced by FastQC (version 0.11.5)