FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005184059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005184059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14195119
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTTAG23050.016.8671285
CCCCTTA34050.013.1097034
CCTTAGA32250.012.5025346
CTTAGAG33000.012.436577
TTAGAGC33450.011.8387628
CACACAA46450.08.9900194
TAGAGCC47650.08.7638339
AGTGAGT29500.08.059141
GTGCACA59400.07.03458261
GTGAGTA33900.06.798922
GCACACA64750.06.67204333
TAACCCC37200.06.5805929
CCTCAGT39850.06.32393033
GATCGGA145500.06.28836251
ACACAAA83300.05.9637915
TGCACAC74900.05.9621042
TCGGAAG160700.05.69030053
ATCGGAA173400.05.48295262
TTAGCAC39352.35068E-85.12333777
CACAAAC87150.05.1222886
Produced by FastQC (version 0.11.5)