FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005184094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005184094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11294822
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA89900.021.2171429
AGAGCAC114600.016.5813568
GATCGGA129850.014.4719051
TCGGAAG141000.013.5948683
AAGAGCA157900.012.4447197
ATCGGAA154500.012.2204492
GGAAGAG198250.09.9844485
AGTCACA39600.08.94321625-29
CGGAAGA224500.08.6347234
GAAGAGC236100.08.5667276
CAGTCAC52550.07.451607725-29
CCAGTCA54950.07.099951325-29
CACACGT59050.06.89917710-14
CACGTCT62000.06.756659510-14
GTCACAT61300.05.894772525-29
TAATCTC57400.05.89457440-44
CTAGATC4602.5204194E-55.6338463140-144
ACACGTC79050.05.499659510-14
GTAATCT64050.05.484856635-39
CGTCTGA77900.05.451529515-19
Produced by FastQC (version 0.11.5)