FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005184139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005184139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15561218
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA54550.012.4051889
ACACTAT64000.012.2607428
AACACTA63800.011.9606677
CACTATA75800.09.9721859
GCCACTC70400.09.7145178
CAAACAC94200.08.5594475
AGCCACT82900.08.3365617
AAACACT100150.07.61991746
AAGCCAC98900.07.06107476
TAGCCCT66700.07.02218772
GCCCTTA72500.06.9565854
TCAAACA132700.06.78695734
ACGTTAA32801.1194061E-85.71130754
ATCAAAC155450.05.70095543
ACCACTA73400.05.6885588
GGCCTAA27050.05.588804275-79
GTTATGT76450.05.26867871
TATTTAG100800.05.2135389
CACCACT77800.05.1817767
ACAAAGC145600.05.047453
Produced by FastQC (version 0.11.5)