FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005184152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005184152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16780001
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA60900.021.9861687
CACTATA64450.020.9989669
ACACTAT66400.020.3818538
AAACACT105300.013.1941946
CAAACAC124050.011.4320315
ACCAAAC127400.011.3074743
CCAAACA133950.010.8050714
CACCCTA34450.08.15196175-79
TATTTAG95250.07.2555329
TACCAAA214700.07.07917452
TAGCCAT110000.07.07234242
GTAGCCA99100.06.84192851
ATACCAA255400.06.25161841
GCCATTA105700.06.19927174
TTAGGCA60350.05.344740460-64
GTACACT60500.05.3315015115-119
CTATAGC59550.05.31908910-14
ACCCTAC57600.05.275658675-79
CCCCTCA59900.05.21611185-89
TACACTC60400.05.1734433115-119
Produced by FastQC (version 0.11.5)