FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005184197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005184197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21080105
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA71150.010.511657
ACACTAT89750.08.8139528
CACTATA105500.07.7026199
CCCTAGG8600.0091505467.5261344
ACCAAAC139150.06.2039033
AAACACT160150.05.6130066
GCCCTTA97550.05.5291954
CCAAACA173750.05.2980134
CAAACAC170150.05.19849165
TAGCCCT94050.05.0505032
Produced by FastQC (version 0.11.5)