FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005184211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005184211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15056992
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTTA40600.023.0984384
TAGAGCC52800.018.414469
CTACCCC43450.017.8721711
CCCTTAG60350.015.6599765
TACCCCT49800.015.3521622
ACCCCTT52500.014.4266323
CGCACAA66550.011.8152714
CTTAGAG105900.09.8781677
TTAGAGC106750.09.3940148
CCTTAGA115550.08.990626
CACAAAC99050.08.8130866
GCGCACA91300.08.21670153
TGCGCAC108300.07.19264032
CAAACTC132450.07.0265498
GTGCGCA108500.07.02332741
AAACTCA149350.06.6547699
GCACAAA124100.06.39466765
ACAAACT161350.06.03627637
CCCCCCA47550.05.359867610-14
AGAGCCC40050.05.28501310-14
Produced by FastQC (version 0.11.5)